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In the present report, we have developed an in vitro system of differentiated pancreatic cells to study the regulation of vasoactive intestinal peptide (VIP) release and synthesis. By using a hybrid cDNA containing the gene of the VPAC-2 receptor, an immortalized rat pancreatic cell line (PRL 25-6) was stably transfected and selected with a SV40 large T antigen. Transfected PRL 25-6 cells expressed in a high level the VPAC-2 receptor, as assessed by Western blot analysis with a specific anti-VPAC-2 receptor antibody. VIP release was studied using a specific radioimmunoassay. The results obtained indicated that VIP is not synthesized by these cells and also that VIP release is under a negative control.Mozart Competition and National Repertory Orchestra

The Mozart Competition and National Repertory Orchestra is an American orchestra based in Boston, Massachusetts. The ensemble was founded in 1984 and makes its home at the Cutler Majestic Theatre at the Carson Center for Performing Arts.

The orchestra is a collaboration of 0b46394aab

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