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We must ever bear in mind what Bible instruction is an importance. Much attention is being given to other lines of work, viz: Engineering, Agriculture, Horticulture Mechanical and Industrial Arts, Law, Medicine and other Professions. This is as it should be. I wish to see greater advancement along all lines till rich ores embedded in the earth by the hand of God are brought forth and converted into palatial structures not second to Solomon's Temple; till the whole earth shall be equal in beauty to the Garden of Eden; till man shall become so skilled in the mechanical and industrial arts that he who performs physical labor will not regard it as a drudgery and something to be dreaded, but the greatest pleasure, the hum of machinery furnishing music that will enchant the soul; till our physicians will become so skilled in medicinal science that physical suffering will be no more.
The pairwise comparison of genetic alterations, including chromosomal alterations and the mutational profile, between leukemia samples obtained at diagnosis and post-transplantation relapse showed an increased number of genomic aberrations after transplantation and in about 10% of patients a deletion of a large homozygous region spanning the whole HLA-locus on chromosome 6p in the relapse sample was observed [27]. Quek and coworkers performed a mutational analysis of disease relapse in a group of allografted AML patients. An increased risk of relapse was observed in AML patients with mutations in WT1, DNMT3A, FLT3-ITD and TP53, whereas IDH1 mutations were associated with a reduced risk of relapse [28]. The serial analysis of the mutational landscape from diagnosis to post-transplantation relapse in AML patients providing evidence that 4/9 patients relapsed with re-expansion of pre-existing clones and 5/9 patients relapsed with evidence of subclonal selection [28]. A recent study based on the analysis of 12 relapsing AML patients provided evidence about a significant gain of WT1 alterations (2/12 pre-transplant and 6/12 post-transplant) and in mutational load [29]. Targeted amplicon sequencing analysis showed that only one of the four cases acquiring new WT1 mutations at relapse seems to be related to the selection of a pre-existing subclone [29].
CD123 is also a diagnostic biomarker of MRD. MRD is an important prognostic factor for relapse and survival in AML and its eradication represents a key therapeutic strategy that may increase the number of AML patients with long-term survival [168]. At the moment, strategies that specifically target MRD are very limited [168]. Interestingly, CD123 represents a diagnostic biomarker of MRD and a potential therapeutic target for MRD eradication. Some trials are specifically evaluating the impact of CD123 targeting on MDR eradication prior to allogeneic stem cell transplantation [168]. 2b1af7f3a8